Persuing Domestic Goddessness
Thursday, August 21, 2003
So now I'm in downtime prepping for more tetrad dissection! and you all thought i was done with that.
well ok (the hell with caps, bah) i guess i should explain what i'm doing. science lesson!
reason #287 why yeast are cool: they exist almost equally as happy as haploids or diploids. haploid is having 1 copy of each gene, like humans have 46 chromosomes, but each one is in 2 copies, meaning we're diploid. yeast can have 1 or 2 copies, and lab strains are just as happy having 1 as having 2, which means they're really easy to mutagenize compared to mice or something). so you take your happy little haploid yeast, mix it with another little happy haploid yeast, they get it on, and voila, you have diploids. but then you can starve them adn they're like, ack starvation, no food, no water, we have to lie low for a while. so they split up into 4 haploid cells (think meiosis, gamete formation) adn have this little funky shell called an ascus covering them. so currently i have taken some starved diploid-turned-haploid cells (now they're tetrads, since there are 4 haploid cells inside each ascus) adn i'm breaking up the ascus so i can play with them. next i'll put the cells on an agar plate and pick up the tetrads with a flat-tipped needle under the microscope, and place each haploid cell on a grid on the plate to see if it grows.
the point of all this: i mated my null strain (the erv26 protein doesn't work) with a bunch of other null strains, and i want to see if the cells containing both defective proteins are dead. fun, eh?
ok now i'm waiting for the plate to dry a bitbefore microscope fun. "fun". actually it's tedious and a little annoying but i'm getting better, it'l probably take me less than an hour today.
so the latest news: i'm trying to buy a condo. trying to get approved for a mortgage makes me feel old. and stupid. ho boy these mortgage loan applications are something else. i've never felt poorer or denser.
but the condo is ok, it's inthe complex where i'm living now, adn we thought i'd save all this money, but now my mortgage payments will be even more than my rent, and that's very icky. so i dunno what to do, i guess i should call my mom and figure it all out. the loan officer guy is ignoring my emails so i'm not sure how he thinks i'm supposed to finish this app. arg. if only i had the full 20% down payment, then things would be easier.
anyone have any money they want to donate to the cause?
So now I'm in downtime prepping for more tetrad dissection! and you all thought i was done with that.
well ok (the hell with caps, bah) i guess i should explain what i'm doing. science lesson!
reason #287 why yeast are cool: they exist almost equally as happy as haploids or diploids. haploid is having 1 copy of each gene, like humans have 46 chromosomes, but each one is in 2 copies, meaning we're diploid. yeast can have 1 or 2 copies, and lab strains are just as happy having 1 as having 2, which means they're really easy to mutagenize compared to mice or something). so you take your happy little haploid yeast, mix it with another little h
Wednesday, August 20, 2003
Well, it’s a been a little while. I meant to write all about Dave and Jenn’s wedding on Monday, but well, that didn’t happen. So sorry for the delay, and here’s the account of it. Ew my space bar doesn’t work so well anymore.
Brandon and I drove (well, I drove, he rode) 5 hours from NH to Rome, NY (we went to Rome for the weekend! Lol) and that was uneventful and pleasant, and we checked into the Vernon Country Suites hotel (the rooms actually were minisuites, it was cool) and then went over to the Gondek’s house on Lake Delta for the rehearsal dinner, which was fun. We saw Brad, who we’d met in February or something, when he drove up with Jenn to visit Dave. And then the next day was the actual wedding, and the church was nice, Jenn looked beautiful, Dave looked good in his fake contacts and tux and the whole get up, the bridesmaids wore mint green, and the ring bearer was so funny, trying to be all serious carrying this little tiny package wrapped in green netting. The only problem was the thunder at the most inopportune moments during the ceremony. But by the time they exchagned vows and blessed the rings, the sun was out.
So then the Dartmouth crew (me, Brandon, Stacey, Jack, Eileen, Brian (honorary D-mouth), Brooke, Craig and Susie) went back to the hotel to enjoy the sounds and sights of K-fest (think radio 104 fest, lots of punk bands on several stages), which included “this is our new song, it’s called “everything sucks”, and naked children running around, oh and a girl that wanted to tell me all about her wedding at St Peter’s Cathedral in NYC while smoking something that probably was not preceded by just another cig.
Then we took the shuttle from the hotel to the Dibble’s Inn for the reception, and that was your basic reception, although we got a nice view of the head table, the dartmouth group and the geneseo group were parallel to the head table, just behind 4 perpendicular tables (all rectangular) of family. The food was good, the cake was good, they smashed it in eachother’s faces but it mostly just made a cake moustache. We all danced, Jenn threw the boquet, Dave threw the garter, we did the electric slide and some cha cha slide that I hadn’t heard of before (Stacey and I tried it), and then Susie met a guy, apparently Jenn’s cousin that lives in Salisbury and works at the school! Oooh… Brian hijacked her camera and took many, many pictures of those two.
And then we went back for karaoke at the hotel, and then Sunday, Susie (who ended up staying with Brandon and me, yeah threesome! Heh) and Brandon and I went to the brunch at the Gondek’s, which was another good time with good food, but this time with a chatty cousin from Salisbury. Heheheh. I’ll feel stupid if he doesn’t call her, then all this heh-heh-ing will be for naught. ;)
So now we’re all back from the wedding. I have more to say, unrelated to the wedding, but I have to dissect some yeast tetrads now, so I’ll write about that lata.
Thursday, August 14, 2003
Wow! I finally have a blog. Some people will be happy.
I feel like I should have something substantial to say in my first entry. Hmm. I'm having lots of thoughts centered around clothing-- I almost ruined this cute lavender polo shirt by throwing it in the wash with a maroon suede hairband tangled in it (got the giant stain out after discovering it and yelling and slamming doors). I can't figure out what to wear to Dave's wedding, which is the day after tomorrow, and I have a new pink belt.
Yesterday a bunch of us moved Rich and Kathi into their new apartment, they now live in Jack's building, which is about 10 minutes away from me. Very cool, especially after their little stint in Claremont, like 45 min away.
Oh I know what I could say. I could introduce myself, or something. .....although, everyone that reads this blog probably knows about it because I sent them the url. So, no introduction. bwahaha
Cookie time! they had chips ahoy sandwiches today. At first I didn't like them, but now I do. I think the best thing about cookie time is that I can try all the hundreds of new cookies they're thinking up without having to buy an entire package. Like Tuesday they had those Oops Oreos, so I had one. It was weird. I prefer regular oreos, and oh boy I can't stand the cappuccino/vanilla oreos. probably because I prefer my capuccinos more bitter than sweet (then they go better with a sweet snack).
Tomorrow I'm driving 5 hrs to upstate NY for Dave's wedding. This will be less than fun. Hopefully the wedding will make up for it (which I'm sure it will). I think us girls are having a fashion show Saturday morning to figure out what to wear. The guys are no help. Honestly, sometimes I miss all girls school.
